HP Labs Technical Reports

A Free-Solution Reactor for Post-Column Fluorescent Detection in Capillary Zone Electrophoresis

Rose Jr., Donald J.

HPL-90-109

Keyword(s):

Abstract: A post-column reactor for fluorescence detection in capillary zone electrophoresis (CZE) is described. The reactor consists of a resevoir of o_-phthaldialdehyde (OPA) reagent which acts as the cathodic resevoir in which the electrophoresis capillary terminates. Zones from the capillary mix and react with the OPA reagent to produce a fluorophore which is detected just beyond the capillary tip. Qualitative and quantitative studies show convective forces as a major source of mixing within the reactor.

Back to Index