HP Labs Technical Reports

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A Free-Solution Reactor for Post-Column Fluorescent Detection in Capillary Zone Electrophoresis

Rose Jr., Donald J.



Abstract: A post-column reactor for fluorescence detection in capillary zone electrophoresis (CZE) is described. The reactor consists of a resevoir of o_-phthaldialdehyde (OPA) reagent which acts as the cathodic resevoir in which the electrophoresis capillary terminates. Zones from the capillary mix and react with the OPA reagent to produce a fluorophore which is detected just beyond the capillary tip. Qualitative and quantitative studies show convective forces as a major source of mixing within the reactor.

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